Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate

In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment release, leading to identification of MADS-box family genes related bud regulation. Thus, aim this study was evaluate a preliminary expression pattern PpDAM13-1 PpDAM13-2 leaf buds pyrifolia Nakai, cultivars Hosui Nijisseiki; Pyrus communis L., Rocha Santa Maria), grown subtropical climate Southern Brazil. Lateral were collected from June September, RNA extracted for evaluation gene by reverse transcription polymerase chain reaction (RT-qPCR). It found that these two decreased as are release dormancy. The cultivar both Nijisseiki showed peaks early June, indicating transition phase. European phase between end-July mid-August. concluded analysis can be used marker changes trees conditions, it suitable assist management strategies overcoming pears’